propidium iodide
propidium iodide
CAS: 25535-16-4
Molecular Formula: C27H34IN4+
propidium iodide - Names and Identifiers
propidium iodide - Physico-chemical Properties
| Molecular Formula | C27H34IN4+ |
| Molar Mass | 541.5 |
| Density | 1.5072 (estimate) |
| Melting Point | 220-225 °C (dec.) (lit.) |
| Water Solubility | Soluble in water at 1mg/ml |
| Solubility | Difficult to mix. |
| Appearance | Flaky Powder |
| Color | Dark red |
| Maximum wavelength(λmax) | ['493 nm, 535 nm'] |
| BRN | 3843838 |
| Storage Condition | 2-8°C |
| Sensitive | Light Sensitive & Hygroscopic |
| MDL | MFCD00011921 |
| In vitro study | Propidium Iodide is a cell-membrane impermeable dye with characteristic excitation maximum at 535 nm and emission maximum at 617 nm which intercalates with nucleic acids with a stoichiometry of one dye per 4-5 base pairs with little sequence preference. Propidium Iodide has evidenced of having no toxic effects on neurons, being today’s most common marker for membrane integrity and cell viability when applied prior to fixation (pre-fixation Propidium Iodide staining method). The pre-fixation staining has been widely used for quantitative assessments of neuronal cell decline in models of acute neurodegeneration, visualized as intensely labeled PI + -pycnotic nuclei of degenerating neurons . Propidium Iodide cannot cross the membrane of live cells, making it useful to measure the percentage of apoptotic cells by flow-cytometric analysis. The flow cytometric data shows an excellent correlation with the results obtained with both electrophoretic and colorimetric methods. This new rapid, simple and reproducible method proves useful for assessing apoptosis of specific cell populations in heterogeneous tissues such as bone marrow, thymus and lymph nodes. |
propidium iodide - Risk and Safety
| Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R68 - Possible risk of irreversible effects R67 - Vapors may cause drowsiness and dizziness R48/20/22 - R40 - Limited evidence of a carcinogenic effect R36/38 - Irritating to eyes and skin. R22 - Harmful if swallowed R20 - Harmful by inhalation R63 - Possible risk of harm to the unborn child |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S22 - Do not breathe dust. S36/37 - Wear suitable protective clothing and gloves. |
| WGK Germany | 3 |
| RTECS | SF7949600 |
| FLUKA BRAND F CODES | 3-8-10-23 |
| HS Code | 29339900 |
propidium iodide - Reference
| Reference Show more | 1. Weng Yahan Bao Weijie Liang Ruimin et al. The effect of MAPK signaling pathway on the proliferation, invasion and migration of endometrial cancer cells [J]. Journal of Clinical and Pathology 2019 000(003):P.470-478. 2. Shi Wenyan Sun Zhen. Synergistic effect and mechanism of ε-polylysine hydrochloride and Nisin on Bacillus cereus [J]. Food and Mechanical 2019 v.35; no.209 (03):21-25 109. 3. Zhang Ke, Deng Qinghua, Ma Shenglin. Effects of Paris polyphylla alcohol extract on proliferation and apoptosis of gastric cancer SGC-7901 cells [J]. Chinese Journal of Traditional Chinese Medicine, 2016. 4. Man, Cai Linheng, Yang Haiping, Yang Xuewen, Si Xiaohui. Effect of DR4 demethylation on proliferation and apoptosis of myeloid leukemia K562 cells [J]. Chinese Journal of Experimental Hematology, 2021,29(02):422-427. 5. Yang, Huirong, et al. "Improvement of multiple-stress tolerance and ethanol production in east duration very-high-gravity fermentation by supplementation of wheel-gluten hydrolysates and their ultrafiltration fractions." Journal of agricultural and food che 6. [IF = 6.953] Yu-Heng Mao et al. "Protection of Bifidobacterial cells against antibiotics by a high molecular weight exopolysaccharide of a medicinal fungus Cs-HK1 through physical interactions." Int J Biol Macromol. 2018 Nov;119:312 7. [IF = 5.279] Huirong Yang et al. "Improvement of Multiple-Stress Tolerance and Ethanol Production in East During Very-High-Gravity Fermentation by Supplementation of Wheat-Gluten Hydrolysates and Their Ultrafiltration Fractions." J Agr Food Chem. 2018;66(39):10233-1024 8. [IF = 4.952] Huirong Yang et al. "Efficient fermentation of very high-gravity worts by brewer's east with wheatgluten hydrolysates and their ultrafiltration fractions supplementations." Lwt Food Sci Technol. 2019 Jun;106:151 9. [IF = 3.31] Xiaoxiao Wang et al. "2D Dendritic Gold Nanostructures Formed on Silicon Nanosheets: Transferability, clean Surface, and their Biomedical Application." Part Part Syst Char. 2018 Oct;35(10):1800268 10. [IF = 15.419] Wei Wang et al. "Gold-Nanoshell-Functionalized Polymer Nanoswimmer for Photomechanical Poration of Single-Cell Membrane." J Am Chem Soc. 2019;141(16):6601-6608 11. [IF = 7.79] Simeng Liu et al. "Adoptive CD8 T-cell grafted with liposomal immunotherapy to counteract the immune suppressive tumor microenvironment and enhance therapy for melanoma." Nanoscale. 2021 Oct;13(37):15789-15803 12. [IF = 7.446] Jun Liu et al. "Novel 2-Substituted 3-Hydroxy-1,6-dimethylpyridin-4(1H)-ones as dual-acting Biofilm Inhibitors of Pseudomonas aeruginosa." J Med Chem. 2020;63(19):10921-10945 13. [IF = 7.086] Yinghan Liu et al. "Inactivation of antibiotic resistant bacterium Escherichia coli by electrochemical disinfection on molybdenum carbide electrode." Chemosphere. 2022 Jan;287:132398 14. [IF = 6.953] Zhou Dong et al. "Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia." Int J Biol Macromol. 2021 Nov;190:101 15. [IF = 4.375] Zou Lei et al. "miR-651-3p Enhances the Sensitivity of Hepatocellular Carcinoma to Cisplatin via Targeting ATG3-Mediated Cell Autophagy." J Oncol. 2021;2021:5391977 16. [IF = 3.463] Yuan-Shan Zhang et al. "Induction of tetraploids in'Red Flash 'caladium using colchicine and oryzalin: Morphological, cytological, photosynthetic and chilling tolerance analysis." Sci Hortic-Amsterdam. 2020 Oct;272:109524 17. [IF = 11.277] Sun Guiling et al. "The maize single-nucleus transcriptome comprehensively describes signaling networks governing movement and development of grass stomata." Plant Cell. 2022 Feb;: |
propidium iodide - Reference Information
| biological applications | Detecting nucleic acids,cancer cells,spores,activity of hydrolase,microorganisms; apoptosis assay; measuring beta-amyloid in Alzheimer's disease (AD); treating diseases associated with androgens,cancer |
| main application | Electroluminescentdisplays; photoresists |
| Introduction | Propidium iodide is a nuclear staining reagent that can stain DNA. It is often used for apoptosis detection. The full English name is Propidium Iodide. It is an analog of ethidine bromide that releases red fluorescence after embedding in double-stranded DNA. |
| biological activity | Propidium Iodide is a red fluorescent dye that can be used for cell staining. |
| Use | Propidium iodide (PI) is an analog of ethidine bromide, which releases red fluorescence after embedding in double-stranded DNA. Although PI cannot pass through the living cell membrane, it can pass through the broken cell membrane and stain the nucleus. PI is often used with fluorescent compounds such as Calcein-AM or FDA to stain living and dead cells at the same time. |
Last Update：2024-04-09 02:00:07
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